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ChIP-Seq, Drosophila Targeted Mutagenesis Featured In Cold Spring Harbor Protocols
High-throughput whole-genome analysis is becoming a standard laboratory approach for investigating cellular processes. Next-generation sequencing is replacing microarrays as the technique of choice for genome-scale analysis, because it offers advantages in both sensitivity and scale. The June issue of Cold Spring Harbor Protocols features "Native Chromatin Preparation and Illumina/Solexa Library Construction" from Keji Zhao and colleagues at the National Heart, Lung and Blood Institute. The article describes sample preparation for sequencing of chromatin-immunoprecipitated DNA (ChIP-Seq) to analyze histone modification patterns using native chromatin and the Solexa/Illumina Genome Analyzer. Step-by-step instructions are given for purification of human CD4+ T cells from lymphocytes and chromatin fragmentation using micrococcal nuclease (MNase) digestion, followed by chromatin immunoprecipitation (ChIP) and construction of a library for sequencing. The article is freely available on the website for Cold Spring Harbor Protocols (http://cshprotocols.cshlp.org/cgi/content/full/2009/6/pdb.prot5237).
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What Is Psychotherapy? What Are The Benefits Of Psychotherapy?
Psychotherapy consists of a series of techniques for treating mental health, emotional and some psychiatric disorders. Psychotherapy helps the patient understand what helps them feel positive or anxious, as well as accepting their strong and weak points. If people can identify their feelings and ways of thinking they become better at coping with difficult situations.
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No Adjustment Method Fully Resolves Confounding By Indication, BUSM Researchers Find
Researchers at Boston University School of Medicine (BUSM) and Boston University School of Public Health have found that no adjustment method fully resolves confounding by indication in observational studies, meaning when the validity of a study is threatened by unmeasured confounding, it is not straightforward to determine which method of adjustment, if any, is most effective in obtaining a valid and precise estimate of effect. The study appears online in the Journal of Clinical Epidemiology.
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AICR And Giant Food Encourage Consumers To Shop For Health, Eat For Life

The American Institute for Cancer Research (AICR) is launching a new campaign in partnership with Giant Food. The campaign, called Shop for Health, Eat for Life, will run in all Giant food stores from June 19 to July 2, 2009. Shop for Health, Eat for Life aims to help individuals and families reduce their risk of cancer, encourage a healthy diet and raise funds for cancer research and education. The campaign encourages Giant customers to purchase specially marked products and offers several in-store promotions and recipes, free nutrition educational materials and product giveaways in select stores. Two to three cents per Shop for Health, Eat for Life item sold will be donated to the AICR, with a goal of contributing up to $150,000 for cancer research. The campaign was announced at AICR"s Washington D.C. headquarters on Wednesday, June 10. Event participants included Kelly Browning, Executive Vice President and CEO of AICR; Robin Michel, Executive Vice President and General Manager of Giant Food; Susan Higginbotham, Ph.D. RD, Director of Research for AICR; and Gerard van der Wulp, Deputy Head of Mission for the Royal Netherlands Embassy. Products included in Shop for Health, Eat for Life are: - Nature"s Promise Whole Wheat Spaghetti 16 oz. - Nature"s Promise Whole Wheat Linguini 16 oz. - Nature"s Promise Premium Organic Spinach 16 oz. - Nature"s Promise Premium Organic Peas 16 oz. - Nature"s Promise Organic Black Beans 15.5 oz. - Nature"s Promise Premium Organic Frozen Strawberries 10 oz. - KRAFT 2% Shredded Cheese - KRAFT Triscuit Baked Whole Grain Wheat Reduced Fat Crackers - KRAFT Planters 16 oz. Dry Roasted Peanuts - KRAFT Caprisun 100% Juices - KRAFT 2% and non-fat Breakstone 16 oz. Cottage Cheese "AICR provides Americans with practical information they can trust -- information that will help them make the everyday choices that lower cancer risk,"said AICR"s Browning. "But they still have to make those choices for themselves, and that"s why we"re so pleased to be a part of this campaign with Giant Food. "Shop for Health, Eat for Life puts AICR"s science-based advice where it can do the most good - right in the grocery aisle, where those all-important choices are actually made every day." A new campaign, Shop for Health, Eat for Life, an initiative of Giant Food and the American Institute of Cancer Research, was announced on Wednesday, June 10, 2009. Kelly Browning, Executive Vice President and CEO of AICR; Robin Michel, Executive Vice President and General Manager of Giant Food and Gerard van der Wulp, Deputy Head of Mission for the Royal Netherlands Embassy, spoke about the relevance of healthy diet and nutritious food choices with cancer prevention. The Shop for Health, Eat for Life program will be in all Giant Food stores from June 19 to July 2, 2009. Giant Chef Mike Reed prepares delicious healthy food options for guests at the Shop for Health, Eat for Life launch event, Wednesday, June 10, 2009 at the American Institute of Cancer Research (AICR). Shop for Health, Eat for Life is a program designed by Giant Food and AICR to educate consumers about cancer prevention and raise funds for research. The program will be in all Giant Food stores from June 19 to July 2, 2009. About Giant Food Giant Food LLC, headquartered in Landover, MD, operates 182 supermarkets in Virginia, Maryland, Delaware and the District of Columbia, and employs approximately 22,000 associates. Included within the 182 stores are 164 full-service pharmacies. For more on Giant visit http://www.giantfood.com. American Institute for Cancer Research


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