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ChIP-Seq, Drosophila Targeted Mutagenesis Featured In Cold Spring Harbor Protocols
High-throughput whole-genome analysis is becoming a standard laboratory approach for investigating cellular processes. Next-generation sequencing is replacing microarrays as the technique of choice for genome-scale analysis, because it offers advantages in both sensitivity and scale. The June issue of Cold Spring Harbor Protocols features "Native Chromatin Preparation and Illumina/Solexa Library Construction" from Keji Zhao and colleagues at the National Heart, Lung and Blood Institute. The article describes sample preparation for sequencing of chromatin-immunoprecipitated DNA (ChIP-Seq) to analyze histone modification patterns using native chromatin and the Solexa/Illumina Genome Analyzer. Step-by-step instructions are given for purification of human CD4+ T cells from lymphocytes and chromatin fragmentation using micrococcal nuclease (MNase) digestion, followed by chromatin immunoprecipitation (ChIP) and construction of a library for sequencing. The article is freely available on the website for Cold Spring Harbor Protocols (http://cshprotocols.cshlp.org/cgi/content/full/2009/6/pdb.prot5237).
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Official Version Of BrainNavigator Launched By Elsevier
Elsevier has announced the launch of the official version of BrainNavigator, a neuroscience research tool developed in collaboration with the Allen Institute for Brain Science and under the editorship of Professor George Paxinos and Charles Watson, Prince of Wales Medical Research Institute, Sydney. After unveiling the prototype version at the Society for Neuroscience"s Neuroscience 2008 tradeshow last November, the rodent brain version is now available at http://www.brainnav.com.
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House Ways And Means Panel Rejects Amendments To Exclude Abortion Coverage From Health Reform
The House Ways and Means Committee on Thursday voted 23-18 to approve its health care reform bill (HR 3200) after rejecting dozens of Republican amendments, including attempts to exclude abortion coverage from the essential benefit package created in the legislation, CQ Today reports. An amendment offered by Rep. Sam Johnson (R-Texas) was rejected in an 18-23 vote; Reps. Bill Pascrell (N.J.), Earl Pomeroy (N.D.) and John Tanner (Tenn.) were the only Democrats to support the amendment. The amendment included exceptions for abortion to save the woman"s life or in cases of rape or incest. Committee members voted 19-22 to reject a similar amendment by House Minority Whip Eric Cantor (R-Va.).The House health reform bill would establish a panel to set a minimum benefits package that health insurers must offer. The bill aims to expand health insurance coverage by mandating that individuals obtain insurance, requiring employers to offer workers coverage or pay a fine, and establishing a health insurance exchange where people could compare and purchase plans. The exchange would include a government-run health insurance option that would compete with private plans (Rubin, CQ Today, 7/17).
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Statement By HHS Secretary Kathleen Sebelius And HHS Agencies On The Signing Of The Family Smoking Prevention And Tobacco Control Act

Today, President Obama took historic action to save lives, reduce health care costs, and help reduce suffering from heart and lung diseases, cancer, and other tobacco-related illnesses. These illnesses kill hundreds of thousands of Americans every year, and the new law gives us the tools to effectively address this major public health issue. This is a key step forward and an important part of health reform. HHS and its agencies are committed to working together to bring a creative, science-based approach to reduce the hazards of smoking and other tobacco use. The implementation of this critical legislation strengthens our public health mission and we will continue to work diligently to protect our children and improve the health of all Americans. For information, please visit: http://www.hhs.gov and http://www.FDA.gov/tobacco. HHS


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